FastOrange® B Agar, 170ml, "Pika"

Guidelines for use
Agar preparation
1. Heat bottle in a 90°C water bath to melt the agar. Alternatively, heat uncapped bottle in a microwave on a low setting until agar has melted.
***Important! Always remove cap before microwaving the bottle, otherwise it might explode!***

2. Prepare Agar:

3. Avoid long holding times for liquid agar, and avoid repeated melting of agar. ***Important! Once melted, agar always should be completely used. Multiple heating or melting should be avoided as the agar will then lose its growth supporting characteristics.***
Never autoclave or sterilize the agar.

Depending on the sample type, the following procedures are recommended:
A. Clear samples (e.g. beer, water, filtered samples) or small volumes of turbid samples

1. Add sample to FastOrange® B Agar plates: - Membrane filters: place filter direct on agar plate, take care not to trap air bubbles between filter and Agar surface. - Liquids: streak direct on Agar plate.

2. Incubate enriched samples at 25 ± 2°C for 2-7 days.

B. Turbid / not filterable samples (e.g. yeast containing beer or fermenter samples)

1. Pour liquid sample into a sterile Petri dish. The sample volume should not exceed 20-30% of the total volume of Petri dish.

2. Add about the double volume of melted Fast Orange® B Agar (kept liquid at 50°C) to the sample.

3. Mix sample and liquid Agar thoroughly by swirling the plate and then let plate cool down to solidify.

4. Incubate enriched samples at 25 ± 2°C for 2-7 days.

Results of visual evaluation

We recommend
Microscopic examination and / or PCR analysis to further characterize and identify bacteria and yeasts.

General information
Store the product in the dark at room temperature (max. 25°C). Cooling below 25°C is NOT necessary. Due to manufacturing, slightly differences in color of culture medium may occur between bottles. This is NOT influencing product quality. Best before date for unopened product is given on the outer label. After opening we cannot guarantee for shelf life.
The product is not suitable for human or animal consumption. It must not be used for the direct propagation of microorganisms which later are used for food production or might get into contact with food.

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