CHROMagar™ Vibrio, 5000mL (373.5 กรัม) 74.7g/L

• Disperse slowly 74.7 g of powder base in 1 L of purified water.

• Stir until agar is well thickened.

• Heat and bring to boil (100 °C) while swirling or stirring regularly.

DO NOT HEAT TO MORE THAN 100 °C. DO NOT AUTOCLAVE AT 121 °C.

Warning 1: If using an autoclave, do so without pressure.

Advice 1: For the 100 °C heating step, mixture may also be brought to a boil in a microwave oven: after initial boiling, remove from oven, stir gently, then return to oven for short repeated bursts of heating until complete fusion of the agar grains has taken place (large bubbles replacing foam).

• Cool in a water bath at 45-50 °C, swirling or stirring gently.

Advice 2: In case of samples with a high presence of Aeromonas, 50 mg of cefsulodin can be added to the mix once cooled down at 45-50 °C (50 mg/L).

• Pour medium into sterile Petri dishes.

• Let it dry and gel.

• Store in the dark before use.

• Prepared media plates can be kept for one day at room temperature.

• Plates can be stored for up to 1 month under refrigeration (2/8 °C) if properly prepared and protected from light and dehydration.