Porphyromonas Gingivalis Agar – P.GING, 4 plates per package (Pre-Order)

Product Description & Summary

P.GING agar is an enriched selective medium used for the isolation and presumptive identification of Porphyromonas gingivalis. The nutritive base consists of casein, heart/meat digest, and yeast extract. The selective agents include colistin, nalidixic acid, and bacitracin at concentrations that inhibit most other organisms. Colistin and nalidixic acid inhibit most aerobic and facultatively anaerobic gram-negative bacteria, while bacitracin is almost exclusively an inhibitor of gram-positive bacteria. This medium is prepared, dispensed, and packaged under oxygen-free conditions to prevent the formation of oxidized products prior to use.

Interpretation of Results & Limitations

If used properly, P.GING agar will support good growth of Porphyromonas gingivalis found in clinical infections.

P.GING agar will not provide complete information for identification of bacterial isolates. Additional test procedures and media are required for complete identification. It is recommended that a non-selective media, such as Brucella Blood Agar (BRU, catalog #: AS-111) also be inoculated from the same clinical specimen to assure recovery of all species present. Consult reference materials for additional information.

Quality Control

The following organisms are routinely used for quality assurance performance testing at Anaerobe Systems.

Product Storage & Shelf Life

Storage: Upon receipt, store at room temperature in original package until used. Avoid overheating or freezing. Do not use media if there are signs of deterioration (shrinking, cracking, or discoloration due to oxidation of media) or contamination. The expiration date applies to the product in its original packaging and stored as directed. Do not use product past the expiration date shown on the label.

Shelf Life: 90 days from date of manufacture.

Formulation*
Pancreatic Digest of Casein     10.00 g
Meat Peptic Digest                     5.00 g
Yeast Extract                              5.00 g
Heart Pancreatic Digest             3.00 g
Corn Starch                                1.00 g
Sodium Chloride                        5.00 g
Agar                                         13.50 g
Vitamin K1 (1.0% solution)        1.00 mL
Hemin (0.1% solution)               5.00 mL
Nalidixic Acid                            15.00 mg
Colistin Methanosulfate            15.37 mg
Bacitracin                                 10.00 mg
Sheep Blood                            50.00 mL
DI Water                                     1.00 L

Final pH: 7.1 ± 0.2 at 25° C
Final weight: 16.0 g ± 1.6 g
*Approximate formula. Adjusted and/or supplemented as required to meet performance criteria.

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